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| Tamieti,B. P.; Damatta,R. A.; Cogo,J. C.; Da Silva,N. S.; Mittmann,J.; Pacheco-Soares,C.. |
| Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell culture; Actin filaments; Nucleus; Endoplasmic reticulum; Crotalus; Rattlesnake venom; Apoptosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100004 |
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| Gallardo-Casas,CA; Guevara-Balcázar,G; Morales-Ramos,E; Tadeo-Jiménez,Y; Gutiérrez-Flores,O; Jiménez-Sánchez,N; Valadez-Omaña,MT; Valenzuela-Vargas,MT; Castillo-Hernández,MC. |
| In Mexico, medicinal plants are widely used. The use of Randia aculeata by healers against snakebites has never been scientifically tested in relation to possible effects on blood parameters and muscle tissue damage. Interviews were carried out in Jamapa, Veracuz, Mexico, with local residents to collect information about the traditional use of Randia aculeata. In this locality, seven pieces of fruit from the plant are mixed in a liter of alcohol, and then administered orally against snakebites. By using histological techniques and a murine model, we explored its cytoprotective properties against the effects of Crotalus simus and Bothrops asper venoms. Possible protections provided by the plant against tissue damage to skeletal and cardiac muscles and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rubiaceae; Antivenoms; Bothrops; Crotalus; Cytoprotection. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300006 |
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| Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC. |
| We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008 |
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| Ferreira Júnior,R.S.; Barravieira,B.. |
| Snake envenoming is a major problem both to veterinary and human medicine in tropical countries due to high incidence, severity, and sequelae. In Brazil, most envenomings involving animals are caused by Bothrops and Crotalus snakes; these are the highest risk to animals. This study reports on Bothrops and Crotalus envenomings in dogs, the main species responsible for epidemiology, pathogenesis, venom action, clinical signs, sequelae and complications, clinical pathology, necropsy findings, diagnosis, and treatment. Veterinarians must be capable of identifying the snake not only by observing its characteristics but also symptom evolution. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snakes; Bothops; Crotalus; Dog. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200002 |
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| Lago,L.A.; Marques Junior,A.P.; Melo,M.M.; Lago,E.P.; Oliveira,N. J. F.; Alzamora Filho,F.. |
| Investigou-se o perfil sorológico de bovinos inoculados com veneno crotálico detoxificado pelo método de iodação e iodação com encapsulação em liposomas. Onze fêmeas com idade média de 18 meses e peso médio de160kg, foram inoculadas com 0,03mg/kg de peso vivo do veneno crotálico do tipo crotamina positivo. Cinco animais receberam o veneno iodado livre, cinco o iodado encapsulado em liposomas e um animal recebeu o mesmo veneno na forma natural, para controle da letalidade da amostra de veneno utilizada. Não houve alterações significativas na concentração de proteínas totais, uréia e creatinina em ambos os tratamentos. Foi observada diferença significativa na concentração de creatinafosfoquinase a partir de duas horas após a inoculação do veneno. A iodação... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Veneno; Crotalus; Bovino. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000500013 |
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| LAGO,Luiz Alberto do; FERREIRA,Paulo Marcos; FACURY FILHO,Elias Jorge; MELO,Marilia Martins; ALZAMORA FILHO,Fernando. |
| Com o objetivo de descrever o quadro clínico de envenenamento crotálico em bovinos, cinco fêmeas, mestiças, com idade variando entre dois e três anos, foram inoculadas com veneno crotálico do tipo crotamina positivo. A dose foi de 0,03 mg/kg de peso vivo. Os animais apresentaram, cronologicamente, o seguinte quadro clínico: na 2ª hora de evolução, apatia, cabeça baixa, letargia profunda e edemaciação discreta no local da inoculação; na 6ª hora, o edema local desapareceu e mioclonias foram observadas nas grandes massas musculares; na 10ª hora, houve diminuição do tônus muscular, de reflexos superficiais, aparecimento de incoordenação motora e decúbito esternal; na 14ª hora, observaram-se movimentos de "pedalagem" e diminuição da sensibilidade profunda; na... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quadro clínico; Envenenamento experimental; Crotalus; Bovino. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962000000400010 |
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| FRANCO,R. L.; ROCHA,C. C.; JORGE,M. T.; RIBEIRO,L.A.. |
| In Brazil, more than 80% of venomous snakebites are caused by Bothrops and about 10% by Crotalus. This study evaluated 133 reported cases that occurred between 1994 and 1996 in the 52 municipalities covered by the Pouso Alegre Regional Health Center in southern Minas Gerais State. Most of the patients were male (89.5%). The most frequently attacked age bracket was that of people in their twenties, and the most frequently bitten anatomical regions were the lower limbs (77.7%), principally the feet (34.6%). Of the 124 cases stating the snake genus, 62.9% were caused by Crotalus and 37.1% by Bothrops. The conclusion of this study is that although the epidemiology of snakebites in Minas Gerais State is similar to other regions of the country, the percentage of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus; Snakebite; Brazil. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000100005 |
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| ROODT,A.R. de; DOLAB,J. A.; SEGRE,L.; SIMONCINI,C.; HAJOS,S. E.; FERNANDEZ,T.; DOKMETJIAN,J. C.; LITWIN,S.; ACCATTOLI,C.; VIDAL,J. C.. |
| The immunochemical reactivity and neutralizing capacity of polyvalent Vipera antivenom (Vipera ammodytes, Vipera aspis, Vipera berus, Vipera lebetina, and Vipera xanthina) were tested on the enzymatic and biological activities of Crotalus durissus terrificus and the following Bothrops venoms from Argentina (Bothrops alternatus, Bothrops ammodytoides, Bothrops neuwiedii, Bothrops jararaca, Bothrops jararacussu, and Bothrops moojeni). The Vipera antivenom reacted weakly when tested by double immunoprecipitation (DIP) and reacted with all the venoms when tested by ELISA. Several components in all the venoms studied were recognized in Western blots. Vipera antivenom deactivated to different degrees in vitro procoagulant, (indirect) hemolytic, and proteolytic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antivenom; Vipera; Bothrops; Crotalus; Cross-reactivity; Cross-neutralization. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000100006 |
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